| : objective:Study on the Therapeutic effect and Mechanism of Guizhi Fuling Pill on reversing Atherosclerotic plaque in ApoE-/-mice.Methods:The atherosclerotic model of ApoE-/-mice was established by high-fat diet, They were divided into model control group, Atto vastatin group, Guizhi Fuling pill group, Atto vastatin + Guizhi Fuling pill group.and Guizhi Fuling pills were given for intervention.Observation of pathomorphological changes of aorta after atherosclerosis by hematoxylin-eosin (HE) staining；And observe the curative effect of plaque.The expression of SM-αaction, transforming growth factor β 1 (TGF-β1) and vascular endothelial growth factor (vascular endothelial growth factor,VEGF) in smooth muscle cells of mouse aortic root plaques were detected by immunohistochemical staining.Results:ApoE-/ mouse model was successfully established: under light microscope, the vascular lumen of ApoE-/- mice was obviously narrowed, protruding plaques could be seen in the lumen, foam cells accumulated in the intima, smooth muscle was disordered, migrated to the intima, and obvious arterial plaques were formed.Effect of atherosclerotic plaque area in ApoE-/- mice: the plaque area in Atto vastatin group decreased, but the difference was not statistically significant (P < 0.01); the plaque area in Guizhi Fuling pill group decreased significantly (P < 0.01), while the plaque area in Guizhi Fuling pill and Atto vastatin group was less (P < 0.01).The influence of positive area of VEGF expression in ApoE-/- mice: there was no VEGF expression in aorta in normal control group; there was little positive expression in Atto vastatin and Guizhi Fuling pill group, but increased in Guizhi Fuling pill group, higher in Atto vastatin group, and the highest in model group.The positive area of VEGF expression in the model group was compared with that in the Guizhi Fuling pill group. There was no positive expression of TGF-β1 and ACTA in all groups of ApoE-/- mice. Conclusion:Compared with Atto vastatin group, Guizhi Fuling pill group significantly reduced the area of arterial plaque, suggesting that Guizhi Fuling pill can improve lumen stenosis, stabilize vascular structure and inhibit vascular remodeling to some extent.Western medicine plus proprietary Chinese medicine can increase the curative effect of reversing plaque. Guizhi Fuling Pill may inhibit the process of atherosclerosis by reducing the expression of VEGF in plaque. |