| 清解承气方对重症急性胰腺炎大鼠肠粘膜屏障保护的实验研究 |
| An experimental study on the intestinal mucosal barrier protection of Qingjie Chengqi Formula in severe acute pancreatitis rats. Liang Ke 1, Wang ning 2 (1.Nanjing University Medical School Affiliated Taikang Xianlin Gonglou Hospital; 2.School of Acupuncture and Tuina, Nanjing University of Chinese Medicine.) |
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| 中文关键词: 重症急性胰腺炎、清解承气方、炎性介质、肠粘膜屏障 |
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| 中文摘要: |
| 【】目的: 探讨清解承气方对重症急性胰腺炎大鼠肠粘膜屏障的保护作用及可能机制。方法: 51只SD大鼠随机等分为假手术组、模型组、清解承气方治疗组。SAP模型制备采用胆胰管逆行注射3％牛磺胆酸钠。造模后观察72h留取标本,记录实验动物的死亡情况,检测肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)和血浆内毒素(Endotoxin)表达的变化,取胰腺组织进行Schmidt病理评分,肠组织作Chiu评分。结果: 各组相比,清解承气方治疗组在降低SAP大鼠的死亡率,下调炎症介质TNF-α、IL-6和内毒素的表达,降低血清淀粉酶活性,减轻胰腺组织水肿、出血、坏死和炎性细胞浸润程度,肠黏膜的损伤程度等方面的作用显著。结论:清解承气方治疗组与模型组相比对SAP的治疗效果明显,其途径可能为清解承气方能够阻断炎症信号通路, 下调炎症介质表达,保护肠粘膜屏障,从而改善SAP病理损伤。 |
| 英文摘要: |
| 【】 Objective: Investigate the protective effect of Qingjie Chengqi Formula on intestinal mucosal barrier in rats with severe acute pancreatitis and its possible mechanism. Methods:51 SD rats were randomly divided into a sham surgery group, a model group, and a Qingjie Chengqi formula treatment group. SAP model was made by 3% sodium taurocholate injected into rats pancreaticobiliary duct. The mortality was registered. The contents of IL-6, TNF-αand endotoxin in blood were tested. The pathological changes of pancreas and intestines had been evaluated. Results: Qingyi Jiedu formula treatment group could significantly decrease mortality, reduce the level of TNF-α,IL-6 and Endotoxin(P<0.05), relieve the pathological changes of pancreas and intestines significantly. Conclusion: Those data with their total score impression suggest that Qingjie Chengqi formula treatment group is better than model group. One possible mechanism is that Qingjie Chengqi Formula can block inflammation signaling pathways and downregulate expression of inflammatory mediators, thereby improving the pathological damage of SAP. |
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