| 结直肠息肉患者薄白苔、白腻苔和黄腻苔的肠道菌群结构特点分析 |
| Analysis of the structural characteristics of gut microbiota in patients with colorectal polyps with thin white fur, white greasy fur, and yellow greasy fur |
| DOI： |
| 中文关键词: 结直肠息肉 舌苔 肠道菌群 16SrDNA基因测序技术 |
| 英文关键词: Colorectal polyps tongue fur Gut microbiota 16S rDNA gene sequencing technology |
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| 中文摘要: |
| 目的 通过分析结直肠息肉(Colorectal Polyps ,CP)患者薄白苔、白腻苔和黄腻苔肠道菌群特征,探讨 CP患者舌苔与肠道菌群之间的关系。方法 研究共招募肠镜检查者87例,从中选取符合要求确诊为CP的患者30例为研究对象,10例肠镜正常者为健康对照组(HC)。其中研究对象分别设置为 CP 薄白苔组(CP_TWF)、CP 白腻苔组(CP_WGF)、CP 黄腻苔组(CP_YGF),每组10例。运用16SrDNA基因组学测序与分析方法,结合生物信息学算法和工具,对HC、CP_TWF、CP_WGF、CP_YGF组肠道菌群的多样性与物种丰度展开分析与比较,探究不同组间肠道菌群的差异与舌苔类型相关性。结果 HC组患者肠道菌群的物种丰度和均匀度最佳,CP_TWF、CP_WGF、CP_YGF组肠道菌群的物种丰度和均匀度依次降低。CP_YGF较HC、CP_TWF、CP_WGF组患者肠道菌群物种的多样性降低,差异有统计学意义(P＜0.05)。HC、CP_TWF、CP_WGF、CP_YGF组患者肠道菌群物种(OTU)数量依次减少。HC、CP_TWF、CP_WGF、CP_YGF组均以厚壁菌门占比最高,并依次呈增长趋势,变形菌门则依次呈减少趋势。属水平上CP_YGF组患者肠道菌群物种丰度与其他组差异较大,CP_TWF组与HC组肠道菌群物种丰度相近。属水平上双歧杆菌(Bifidobacterium)在HC相对丰度最高,在CP_YGF组相对丰度明显减低(P＜0.05)。CP_YGF组肠道菌群中另枝菌属(Alistipes)、优真杆菌属(Eubacterium)、脱硫弧菌属(Desulfovibrio)、瘤胃球菌属(Ruminococcaceae)、丁酸单胞菌属(butyricimonas)较其他三组增多(P＜0.05)。结论:CP薄白苔、白腻苔、黄腻苔患者肠道菌群丰富度和多样性均较健康者下降,黄腻苔下降最明显,黄腻苔患者肠道致病性细菌增多而具有保护作用的细菌明显减少；为中医药防治肠息肉疾病复发、癌变提供客观依据。 |
| 英文摘要: |
| Objective: The aim is to delve into the relationship between tongue fur and gut microbiota in patients with colorectal polyps (CP). This is achieved by analyzing the characteristics of gut microbiota in patients presenting with thin white fur, white greasy fur, and yellow greasy fur. Methods: A total of 87 colonoscopy examinees were recruited for this study. From these, 30 patients diagnosed with CP who met the requirements were selected as the research subjects, and 10 patients with normal colonoscopy findings were chosen as the healthy control group (HC). The research subjects were further divided into the CP thin white fur group (CP_TWF), CP white greasy fur group (CP - WGF), and CP yellow greasy fur group (CP_YGF), with 10 cases in each group. Employing 16S rDNA genomics sequencing and analysis methods, in combination with bioinformatics algorithms and tools, the diversity and species abundance of gut microbiota in the HC, CP_TWF, CP_WGF, and CP_YGF groups were analyzed and compared. This was done to explore the differences in gut microbiota among different groups and their correlations with various tongue - coating types.It was found that the species abundance and evenness of gut microbiota in the HC group were optimal. In contrast, the species abundance and evenness of gut microbiota in the CP_TWF, CP_WGF, and CP_YGF groups decreased in sequence. The diversity of gut microbiota species in the CP_YGF group was significantly lower compared to the HC, CP_TWF, and CP_WGF groups (P<0.05). The number of gut microbiota species (OTUs) decreased successively in the HC, CP_TWF, CP_WGF, and CP_YGF groups. In all the HC, CP_TWF, CP_WGF, and CP_YGF groups, Firmicutes had the highest proportion, and this proportion showed an increasing trend, while Proteobacteria showed a decreasing trend. At the genus level, the abundance of gut microbiota species in the CP_YGF group differed remarkably from that in other groups, while the abundance of gut microbiota species in the CP_TWF group was similar to that in the HC group. In the HC group, Bifidobacterium had the highest relative abundance at the genus level, but its relative abundance was significantly reduced in the CP_YGF group (P<0.05). Compared with the other three groups, the gut microbiota of the CP_YGF group exhibited an increase in the genera Alistipes, Eubacteria, Desulfovibrio, Ruminococcus, and Butyricimonas (P<0.05). Conclusion: The richness and diversity of gut microbiota in patients with CP presenting with thin white fur, white greasy fur, and yellow greasy fur are all lower than those in healthy individuals, with the most pronounced decrease observed in the yellow greasy fur group. Patients with yellow greasy fur have an increase in pathogenic bacteria in their gut and a significant reduction in protective bacteria. This study provides an objective basis for the prevention and treatment of the recurrence and carcinogenesis of intestinal polyp disease using traditional Chinese medicine. |
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