文章摘要
基于网络药理学的丹参-红花药对治疗紧张型头痛 的作用机制研究
Study on the Mechanism of Herb Pair Radix Salviae Miltiorrhizae-Flos Carthami in Treating Tension-type Headache Based on the Network PharmacologyXu Xiangqian1,2 Zhang Xiao3 Ding Yanbing1,2*
投稿时间:2021-04-04  修订日期:2021-04-12
DOI:
中文关键词: 网络药理学  丹参-红花  紧张型头痛  信号通路  作用机制
英文关键词: Network Pharmacology  Danshen-Honghua  Tension-type headache  Signal Pathway  Mechanism of action
基金项目:国家中医药管理局中医临床研究基地业务建设科研专项资助项目(No.JDZX2015295);中医药脑病循证能力提升及平台建设(2019XZZX-NB003)
作者单位邮编
许项前 湖北省中医院 430070
章消 湖北中医药大学 
丁砚兵 湖北省中医院 430070
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中文摘要:
      目的 使用网络药理学技术方法初步探讨丹参-红花药对治疗紧张型头痛(Tension-type Headache,TTH)的作用机制。方法 利用数据库TCMSP筛选出丹参和红花的有效活性成分,并预测活性成分的靶点;在NCBI-gene、GeneCards、Drugbank数据库中检索TTH疾病的相关靶点;使用Biogenet构建蛋白质-蛋白质互相作用(PPI)网络图,并筛选交集网络中丹参-红花治疗TTH的关键靶点;用Metascape对最后得到的靶点进行生物通路和富集分析。结果 得到87个药物有效成分,对应233个药物靶点;70个TTH疾病靶点;筛选后的关键靶点共168个,266条KEGG通路。丹参和红花治疗TTH丹参-红花治疗TTH的主要成分可能是丹参酮ⅡA、木犀草素、槲皮素、山柰酚等,关键靶点包括ESR1、NTRK1、APP等,涉及到的关键信号通路有PI3K-Akt信号通路、MAPK信号通路、FoxO信号通路、神经营养蛋白信号通路等。结论 初步证明丹参-红花治疗TTH的作用机制是多成分、多靶点、多途径的,与神经细胞的生长、增殖、分化,神经递质的释放、氧化应激以及炎症反应等生物过程有关联。
英文摘要:
      Objective To preliminarily explore the mechanism of Radix Salviae Miltiorrhizae-Flos Carthami herb pair on the treatment of Tension-type Headache (TTH) using network pharmacology.Methods Use the TCMSP to screen out the effective active ingredients of Danshen-Honghua,and predict the targets of the active ingredients;search the relevant targets of TTH disease in NCBI-gene, GeneCards and Drugbank databases;Use Biogenet to construct a protein-protein interaction (PPI) network diagram, and screen the key targets of Danshen-Honghua in the intersection network for the treatment of TTH; use Metascape to analyze the biological pathway and enrichment of drug-disease intersection targets.Results 87 drug active ingredients were obtained, corresponding to 233 drug targets; 70 TTH disease targets; a total of 168 key targets and 266 KEGG pathways were screened. Salvia and safflower in the treatment of TTH Danshen-Honghua in the treatment of TTH may be the main components of tanshinone ⅡA, luteolin, quercetin, kaempferol, etc. The key targets include ESR1, NTRK1, APP, etc., and the key signal pathways involved There are PI3K-Akt signaling pathway, MAPK signaling pathway, FoxO signaling pathway, neurotrophin signaling pathway, etc.Conclusion It is preliminarily proved that the mechanism of action of Danshen-safflower in the treatment of TTH is multi-component, multi-target, and multi-pathway, which is related to the growth, proliferation, differentiation of nerve cells, the release of neurotransmitters, oxidative stress, and inflammation Associated.
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