| 白藜芦醇对糖尿病大鼠肾脏JAK/STAT信号通路的影响 |
| Effects of resveratrol on renal JAK/STAT signaling pathway in diabetes rats |
| DOI： |
| 中文关键词: 白藜芦醇 糖尿病 大鼠 肾脏 JAK/STAT信号通路 |
| 英文关键词: Resveratrol Diabetes Rats Kidney JAK/STAT signaling pathway |
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| 中文摘要: |
| 目的:研究白藜芦醇对糖尿病大鼠肾脏JAK/STAT信号通路的影响。方法:高糖高脂饲料喂养大鼠8w后,用链脲佐菌素45mg/kg腹腔注射造模。将成模大鼠随机分为糖尿病组、白藜芦醇低剂量组(10mg/kg)、白藜芦醇中剂量组(20mg/kg)、白藜芦醇高剂量组(40mg/kg)。连续4周灌胃给药治疗,分别检测各组大鼠血糖、血清胆固醇、甘油三酯、尿素氮和肌酐；ELISA法检测血清ICAM-1和IL-6水平；HE法观察肾脏的形态学改变；PCR法检测肾组织JAK2、STAT3、SOCS1、ICAM-1和IL-6的mRNA水平；Western blot法检测p-JAK2、p-STAT3、SOCS1、ICAM-1和IL-6在肾组织中的表达。结果:与模型组相比,白藜芦醇各组大鼠的血糖、血清胆固醇、甘油三酯、尿素氮和肌酐均降低,并呈现剂量依赖效应,以白藜芦醇高剂量组变化最为明显。糖尿病大鼠经白藜芦醇治疗后,肾脏病理损伤减轻,血清ICAM-1和IL-6显著降低,肾组织JAK2、STAT3、ICAM-1和IL-6的mRNA表达和蛋白表达均降低,而SOCS1的mRNA表达和蛋白表达均上升,尤以白藜芦醇高剂量组最为明显。结论:白藜芦醇能够抑制糖尿病大鼠肾JAK/STAT信号通路,通过提高SOCS1水平,抑制JAK2和STAT3的活化,从而减少炎症因子ICAM-1和IL-6的表达。 |
| 英文摘要: |
| Objective: To study the effect of resveratrol on renal JAK/STAT signaling pathway in diabetes rats. Methods: After feeding rats with high sugar and high fat feed for 8 weeks, streptozotocin 45mg/kg was intraperitoneally injected to create a model. The model rats were randomly divided into diabetes group, low dose resveratrol group (10mg/kg), medium dose resveratrol group (20mg/kg), and high dose resveratrol group (40mg/kg). Continuous 4 weeks of gastric administration for treatment, blood glucose, serum cholesterol, triglycerides, urea nitrogen, and creatinine were measured in each group of rats; and ELISA method was used to detect the levels of serum ICAM-1 and IL-6; and HE staining was used to observe the pathological changes of the kidneys; and fluorescence quantitative PCR was used to detect the mRNA levels of JAK2、STAT3、SOCS1、ICAM-1 and IL-6 in renal tissue; and western blot was used to detect the expression of p-JAK2、p-STAT3、SOCS1、ICAM-1 and IL-6 in renal tissue. Results: Compared with the model group, the blood glucose, serum cholesterol, triglycerides, urea nitrogen, and creatinine of rats in each group of resveratrol decreased in a dose-dependent manner, with the highest dose group showing the most significant changes. After resveratrol treatment, renal pathological damage in diabetes rats was reduced, and serum ICAM-1 and IL-6 were significantly reduced, and the mRNA and protein expression of JAK2, STAT3, ICAM-1, and IL-6 in renal tissue were reduced, while the mRNA and protein expression of SOCS1 increased, especially in the high-dose group of resveratrol. Conclusion: Resveratrol can inhibit the renal JAK/STAT signaling pathway in diabetes rats, and inhibit the activation of JAK2 and STAT3 by increasing the level of SOCS1, thereby reducing the expression of inflammatory factors ICAM-1 and IL-6. |
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