| 利用AFLP指纹技术研究中国沿海真鲷群体的遗传变异和趋异 |
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| 引用本文： | 王志勇.利用AFLP指纹技术研究中国沿海真鲷群体的遗传变异和趋异[J].水产学报,2001,25(4):289-293,T001. |
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| 作者姓名： | 王志勇 |
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| 作者单位： | 1. 集美大学水产学院,
2. 东京水产大学资源育成学科, |
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| 基金项目： | 农业部九五渔业规划重点项目(渔95-A-96-01-02). |
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| 摘 要： | 用AFLP指纹技术在中国沿海真鲷群体进行遗传学变异和趋异分析，使用了6对引物：E－ACC／M－CAT、E－AGG／M－CTG、E－AGG／M－CTT、E－AAC／M－CTA、E－ACA／M－CTG、E－AAC／M－CAA，在威海、厦门和北部海湾海区三个野生群体区42个体中检出646个扩增位点（片段大小50－400bp)，其中70％位点在群体内或群体间呈现多态。三个群体的多态位点比例变化在58．4％－64．0％，遗传相似系数变化在0．8425－08200，以威海群体的遗传变异量最大，北部湾群体的最小。三群体空遗传距离和UPGMA谱系图显示，厦门海区与北部湾的真鲷差异较小，可以认为是同一个亚种群的不同群体，黄海真鲷与前二个群体差异比较大，属于另一个不同的亚种群。
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| 关 键 词： | 真鲷 群体遗传 扩增片段长主多态性指纹 遗传变异 趋异 海水鱼 |
| 文章编号： | 1000-0615(2001)04-0289-06 |
| 收稿时间： | 2014/4/15 0:00:00 |
| 修稿时间： | 2014/4/15 0:00:00 |
Genetic variation and divergence of Pagrus major from China seas using AFLP fingerprinting |
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| WANG Zhi-yong.Genetic variation and divergence of Pagrus major from China seas using AFLP fingerprinting[J].Journal of Fisheries of China,2001,25(4):289-293,T001. |
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| Authors: | WANG Zhi-yong |
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| Affiliation: | WANG Zhi-yong1,WANG Yi-lei1,LIN Li-min1,HONG Hui-xin1,ZHANG Ya-zhi1,KHOO Sock Kean2,OKAMOTO Nobuaki2 |
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| Abstract: | AFLP fingerprinting was used to detect genetic variation and divergence of three wild stocks of Red Sea Bream, Pagrus major, from the Yellow Sea (Weihai), the East China Sea (Xiamen) and the South China Sea (Beibuwan). A total of 42 individuals were studied using six primer pairs (E-ACC/M-CAT, E-AGG/M-CTG, E-AGG/M-CTT, E-AAC/M-CTA, E-ACA/M-CTG and E-AAC/M-CAA). 452 (70.0%) of 646 selectively amplified fragments ranging in size from 50 bp to 400 bp were polymorphic. The mean percentages of polymorphic bands of the three geographic stocks in question varied from 58.4% to 64.0%, and the genetic similarities estimated within stocks varied from 0.8425 to 0.8200. The maximum and the minimum polymorphism occurred in the samples from the Yellow Sea population and the South China Sea population, respectively. The genetic distances between stocks and UPGMA dendrogram indicated that the genetic divergence between the East China Sea stocks and the South China Sea stocks of P.major was small, suggesting that they belong to the same subpopulation, and the Yellow Sea stocks was separated from the other two stocks, belonging to a different subpopulation. |
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| Keywords: | Pagrus major population Amplified Fragments Length Polymorphism fingerprinting(AFLP) genetic variation and divergence |
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