| 3个仿刺参地理种群遗传变异的微卫星DNA分析 |
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| 引用本文： | 谭杰.3个仿刺参地理种群遗传变异的微卫星DNA分析[J].水产学报,2007,31(4):437-442. |
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| 作者姓名： | 谭杰 |
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| 作者单位： | 1. 中国水产科学研究院黄海水产研究所,山东,青岛,266071;上海水产大学生命科学与技术学院,上海,200090
2. 中国水产科学研究院黄海水产研究所,山东,青岛,266071 |
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| 基金项目： | 山东省科技攻关项目;农业结构调整技术专项基金 |
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| 摘 要： | 运用微卫星DNA技术对仿刺参烟台群体、威海群体、大连群体的3个野生群各20个个体进行了遗传分析。对9个基因位点进行了扩增,共获得了59个等位基因。评估了9对微卫星引物的多态信息含量(PIC),范围在0.5129~0.8794之间。结果表明:3个野生群体的平均杂合度观测值分别为0.6416、0.6595、0.5824,平均杂合度期望值分别为0.7641、0.7161、0.7364;在Hardy-Weinberg平衡条件下,进行了P检验,发现3个群体均有位点发生了显著偏离;对3个群体进行了配对Fst值计算,发现3个群体之间遗传分化较弱。从变异贡献率来看,95.68%的变异来自个体之间,4.32%的变异来自群体之间。经过聚类分析,发现威海群体和大连群体之间亲缘关系较近,烟台群体与前两群体亲缘关系较远。
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| 关 键 词： | 仿刺参 地理群体 遗传变异 微卫星DNA |
| 文章编号： | 1000-0615（2007）04-0437-06 |
| 收稿时间： | 2007/7/16 0:00:00 |
| 修稿时间： | 2006-11-08 |
Genetic diversity of three geographical populations of Apostichopus japonicus revealed by microsatellite DNA |
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| TAN Jie.Genetic diversity of three geographical populations of Apostichopus japonicus revealed by microsatellite DNA[J].Journal of Fisheries of China,2007,31(4):437-442. |
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| Authors: | TAN Jie |
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| Affiliation: | Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China |
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| Abstract: | The microsatellite DNA technique was applied to assess genetic diversity and genetic differentiation in three wild populations of Apostichopus japonicus from Yantai,Weihai and Dalian.9 polymorphic loci were screened electrophoretically for genetic variation of the three wild populations.The following parameters were calculated: heterozygosity,polymorphism information content(PIC),effective allele number,allele frequency,genetic distance,genetic identity coefficient,Hardy-Weinberg balance deflection index and so on.Results showed that the number of total alleles of these 9 microsatellite loci was 59.The average of allele number in each microsatellite locus of the Yantai,Weihai and Dalian populations was 6.5556,5.8889 and 6.0000,respectively;the average effective allele number was 4.6033,4.0466 and 4.1367,respectively;The PIC of polymorphic loci varied between 0.5129-0.8794.The average observed heterozygosity(Ho) of three populations was 0.6416,0.6595 and 0.5824 respectively,and the average expected heterzygosity(He) was 0.7641,0.7161 and 0.7364,respectively.Cluster analysis of the three wild populations performed with UPGMA(unweighted pair group method with arithmetic mean) confirmed the closer linkage between Weihai and Dalian than their individual distances to Yantai. |
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| Keywords: | Apostichopus japonicus geographic populations genetic diversity microsatellite DNA |
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